Plasmid

Part:BBa_K3179104

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-16)


hEF1a-rtTA3

hEF1α-rtTA3


Usage and Biology

This part consists with promoter hEF1α and basic part rtTA3. hEF1α is a human elongation factor 1α gene promoter and is a strong mammalian promoter. hEFα is usually used in regular expression and has a stable expressional level, which is independent with cell type. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.


T-SYSU-CHINA-pCMEhEF1a rtTA.jpg

In our experiment, hEFα-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None